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Updated: Jun 9, 2026

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity
Published on: January 29, 2018
Modification of human papillomavirus minor capsid protein L2 by sumoylation
Martina Bergant Marusic1, Nina Mencin, Mia Licen
1University of Nova Gorica, Vipavska 13, SI-5000 Nova Gorica, Slovenia. martina.bergant@ung.si
Abstract:
The human papillomavirus (HPV) minor capsid protein L2 plays important roles in the generation of infectious viral particles and in the initial steps of infection. Here we show that HPV-16 L2 protein is sumoylated at lysine 35 and that sumoylation affects its stability. Interestingly, the sumoylated form of L2 cannot bind to the major capsid protein L1, suggesting a mechanism by which capsid assembly may be modulated in an infected cell. Additionally, L2 appears to modulate the overall sumoylation status of the host cell. These observations indicate a complex interplay between the HPV L2 protein and the host sumoylation machinery.
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