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Related Concept Videos

Standards of Care II01:19

Standards of Care II

Nurses bear specific legal responsibilities under several federal statutes, including:
Standards of Care I01:22

Standards of Care I

Federal statutes profoundly impact nursing practice, providing critical guidelines to ensure patient care is equitable, accessible, and of the highest quality. The following laws address distinct aspects of healthcare provision and patient rights:
Cancer Therapies02:49

Cancer Therapies

Cancer therapies are various modes of treatment, such as surgery, radiation therapy, and chemotherapy that are administered to cancer patients.
However, cancer treatments can pose several challenges, as therapies used to kill cancer cells are generally also toxic to normal cells. Moreover, cancer cells mutate rapidly and can develop resistance to chemical agents or radiation therapy. Besides, all types of cancer cells may not respond to the same therapy. Some cancer cells respond to one...
Cancer Therapies02:49

Cancer Therapies

Cancer therapies are various modes of treatment, such as surgery, radiation therapy, and chemotherapy that are administered to cancer patients.
However, cancer treatments can pose several challenges, as therapies used to kill cancer cells are generally also toxic to normal cells. Moreover, cancer cells mutate rapidly and can develop resistance to chemical agents or radiation therapy. Besides, all types of cancer cells may not respond to the same therapy. Some cancer cells respond to one...
Cancer Vaccines01:30

Cancer Vaccines

Cancer treatment vaccines are a rapidly evolving field that offers a promising approach to immunotherapy. Unlike traditional vaccines that prevent diseases, cancer treatment vaccines are designed to treat existing cancers by stimulating the immune system to recognize and attack cancer cells.
Cancer vaccines come in two categories: preventive (prophylactic) and treatment (active). Preventive vaccines, such as the Human Papillomavirus (HPV) vaccine, protect against viruses that cause certain...
Treatment Resistant Cancers02:56

Treatment Resistant Cancers

Cancer is the second leading cause of death in the United States. A cancer cell is genetically unstable and hence can mutate faster. They can also modify their microenvironment and escape immune surveillance. The difficulties in treating cancer are further compounded by the emergence of rapid resistance to anticancer drugs. The most common ways to attain resistance in cancer cells include alteration in drug transport and metabolism, modification of drug target, elevated DNA damage response, or...

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Related Experiment Video

Updated: Jun 9, 2026

Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells
08:28

Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells

Published on: May 9, 2025

[HER2 ASCO guidelines. The answer to everything?].

E Burandt1, G Sauter

  • 1Institut für Pathologie, Universitätsklinikum Hamburg-Eppendorf, Martinistr. 52, 20246 Hamburg. e.burandt@uke.uni-hamburg.de

Der Pathologe
|August 27, 2010
PubMed
Summary
This summary is machine-generated.

HER2 testing is crucial for breast cancer treatment decisions. Fluorescence in situ hybridization (FISH) is recommended over immunohistochemistry (IHC) for accurate HER2 gene status determination, improving targeted therapy response prediction.

More Related Videos

Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers
11:34

Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers

Published on: December 5, 2017

Related Experiment Videos

Last Updated: Jun 9, 2026

Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells
08:28

Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells

Published on: May 9, 2025

Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers
11:34

Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers

Published on: December 5, 2017

Area of Science:

  • Oncology
  • Molecular Diagnostics
  • Genetics

Background:

  • HER2 gene amplification occurs in 15-20% of breast cancers, necessitating routine testing.
  • Accurate HER2 testing is vital for guiding targeted therapies like trastuzumab and lapatinib.

Purpose of the Study:

  • To evaluate current HER2 testing methodologies in breast cancer.
  • To address issues within the ASCO-CAP guideline recommendations for HER2 testing.
  • To compare the accuracy and reliability of IHC versus FISH for HER2 determination.

Main Methods:

  • Review of current literature and guideline recommendations.
  • Analysis of laboratory testing considerations including accuracy, reproducibility, and precision.
  • Evaluation of biological and technical factors impacting HER2 protein analysis.

Main Results:

  • FISH methods demonstrate superior accuracy, reproducibility, and precision compared to IHC for HER2 status determination.
  • FISH is less susceptible to tissue fixation artifacts, ensuring more consistent results.
  • FISH results show a stronger correlation with patient response to HER2-targeted therapies.

Conclusions:

  • FISH is recommended as the primary HER2 testing method for breast cancer patients eligible for targeted therapies.
  • FISH offers greater reliability and predictive value for treatment response than IHC.
  • Further advancements are needed for alternative methods to match FISH's accuracy and predictive capabilities.