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10:18
Concurrent Quantification of Cellular and Extracellular Components of Biofilms
Published on: December 10, 2013
Microbial contamination and inhibitory effect against Streptococcus mutans from fifth-generation bonding systems
Sérgio L Pinheiro1, Herbert H Soares, Mariângela C Ribeiro
1PUC-Campinas Dental School, Campinas, São Paulo, Brazil. slpinho@puc-campinas.edu.br
Journal of Applied Biomaterials & Biomechanics : JABB
|August 27, 2010
Summary
Two dental bonding systems, Single Bond (SB) and Excite (EX), showed microbial contamination and an inhibitory effect against Streptococcus mutans (SM). This effect persisted even after 20 applications, unlike Prime & Bond (PB).
Area of Science:
- Dental Materials Science
- Microbiology
- Biocompatibility
Background:
- Dental bonding systems are crucial in restorative dentistry.
- Microbial contamination of bonding agents can impact oral health.
- Streptococcus mutans (SM) is a primary cariogenic bacterium.
Purpose of the Study:
- To assess microbial contamination in Prime & Bond (PB), Single Bond (SB), and Excite (EX) bonding systems.
- To evaluate the in vitro inhibitory effect of these bonding systems against Streptococcus mutans (SM).
- To determine the persistence of these effects after repeated applications (10 and 20).
Main Methods:
- Bonding materials were applied to brain-heart infusion broth and cultured on blood agar.
- Inhibitory effects were tested by placing material-infused filter discs on SM-seeded agar plates.
- Statistical analysis included Cochran and Kruskal-Wallis tests to compare bacterial counts and inhibitory effects.
Main Results:
- Single Bond (SB) and Excite (EX) exhibited significant microbial contamination and inhibitory effects against SM (p<0.05).
- Prime & Bond (PB) showed statistically significant differences compared to SB and EX.
- The inhibitory capacity of SB and EX against SM remained significant after 20 applications.
Conclusions:
- Variations in monomer composition, solvents, and application techniques influence bacterial contamination and SM inhibition.
- SB and EX demonstrate potential for managing SM in a clinical setting.
- Further research is needed to correlate in vitro findings with clinical outcomes.
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