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Published on: October 31, 2013
Centromeric DNA of Kluyveromyces lactis
J J Heus1, B J Zonneveld, H Y Steensma
1Department of Cell Biology and Genetics, Leiden University, The Netherlands.
Abstract:
A direct selection method was used to isolate centromeres from a genomic library of the yeast Kluyveromyces lactis. The method is based on the lethality at high copy number of the ochre-suppressing tRNA gene SUP11. Five different chromosomal fragments were found that confer mitotic stability to plasmids containing a replication origin of K. lactis (KARS). In addition, KARS plasmids containing these fragments have a copy number of approximately one, and each of the five fragments hybridizes to a different chromosome of K. lactis. From these results we conclude that five of the six centromeres of K. lactis have been isolated. These centromeres do not function in S. cerevisiae.
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