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Red algal plasmids.

L J Goff1, A W Coleman

  • 1Department of Biology, University of California, Santa Cruz 95064.

Current Genetics
|December 1, 1990
PubMed
Summary
This summary is machine-generated.

Circular DNA plasmids were identified in five red algal genera. In Gracilariopsis lemaneiformis, these plasmids replicate autonomously, are transcribed, and may be useful for genetic transformation.

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Area of Science:

  • Marine Biology
  • Molecular Biology
  • Genetics

Background:

  • Circular double-stranded DNA (dsDNA) plasmids are known in various organisms.
  • Their presence and function in red algae (Rhodophyta) remain largely unexplored.
  • Understanding algal plasmids is crucial for their potential biotechnological applications.

Purpose of the Study:

  • To investigate the occurrence and characteristics of circular dsDNA plasmids in red algae.
  • To analyze the nature and potential function of plasmids in Gracilariopsis lemaneiformis.
  • To assess the utility of red algal plasmids as vectors for genetic engineering.

Main Methods:

  • Screening of 21 red algal genera for circular dsDNA plasmids.
  • Plasmid DNA isolation, cloning, and sequencing from Gracilariopsis lemaneiformis.

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  • DNA-DNA hybridization assays to determine genomic relationships.
  • Northern blot analysis to detect RNA transcripts.

    • Main Results:

    • Circular dsDNA plasmids were found in 5 out of 21 red algal genera.
    • Gracilariopsis lemaneiformis harbors two distinct plasmids (4.4 and 3.5 kbp) that do not cross-hybridize with host genomes or other algal DNAs.
    • These plasmids are present in high copy numbers, replicate autonomously, and exhibit specific transcription, indicating functional relevance.
    • Two potential open reading frames were identified on the 3.5 kbp plasmid.

    Conclusions:

    • Red algae possess circular dsDNA plasmids with unique genetic characteristics.
    • The identified plasmids in G. lemaneiformis are actively transcribed and stably maintained, suggesting a significant biological role.
    • These plasmids represent promising candidates for developing novel vectors for the genetic transformation of economically important red algal species.