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[Identification of myosin light chain 1 from human heart ventricles on two-dimensional electrophoretograms using
Two-dimensional electrophoresis performed according to O'Farrell was used to isolate the myosin light chain I from human heart ventricles. This chain was further used to obtain hybridomas able to secrete monoclonal antibodies. This method permits to increase the yield of monoclonal antibody-secreting hydridomas and to spare effort with regard to cultivation and testing of experimental samples.
Two-dimensional electrophoresis performed according to O'Farrell was used to isolate the myosin light chain I from human heart ventricles. This chain was further used to obtain hybridomas able to secrete monoclonal antibodies. This method permits to increase the yield of monoclonal antibody-secreting hydridomas and to spare effort with regard to cultivation and testing of experimental samples.