Hormonal regulation of cultured rabbit endometrial cells

A technique for culturing primary explants of rabbit endometrial cells in chemically defined medium has been developed. Diethylstilbestrol and natural estrogens were found to increase the rate of DNA initiation while progesterone had the opposite effect. Ultrastructural studies revealed that with estrogens, the cultures had the appearance of rapidly dividing cells having large euchromatic nuclei and prominent nucleoli, with aboundant free ribosomes in the cytoplasm. On the other hand, progesterone induced the formation of large multinucleated cells and also converted the cells to a more secretory type. When the cultures appear secretory, a protein called blastokinin (uteroglobin) is believed secreted. We have found that cultured cells synthesize blastokinin and progesterone is necessary for its continuous synthesis. The cultures were found to have receptors for the ovarian hormones and these receptors exhibited saturation kinetics. The dissociation constants for the receptors were also determined. A hypothetical model for the hormonal regulation of cell proliferation and differentiation is proposed.