Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Preparative free-flow isoelectric focusing: modeling and experiments.

M Poux1, J Bertrand

  • 1Laboratoire de Génie Chimique, URA CNRS, Toulouse, France.

Electrophoresis
|November 1, 1990
PubMed
Summary

Preparative free-flow isoelectric focusing effectively separated human serum albumin and beta-lactoglobulin. Chemical engineering principles and a numerical model validated the separation process for these proteins.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pharmacokinetic interaction between rifampicin and clindamycin in staphylococcal osteoarticular infections.

International journal of antimicrobial agents·2023
Same author

Development of 3D-QSAR and pharmacophoric models to design new anti-<i>Trypanosoma cruzi</i> agents based on 2-aryloxynaphthoquinone scaffold.

SAR and QSAR in environmental research·2022
Same author

Aspect ratio of nano/microstructures determines Staphylococcus aureus adhesion on PET and titanium surfaces.

Journal of applied microbiology·2021
Same author

MMP-9 mediated Syndecan-4 shedding correlates with osteoarthritis severity.

Osteoarthritis and cartilage·2020
Same author

[Coronary aneurysm, it exists !]

Revue medicale de Liege·2020
Same author

Population pharmacokinetic modeling of sustained release lithium in the serum, erythrocytes and urine of patients with bipolar disorder.

European journal of clinical pharmacology·2018

Area of Science:

  • Biochemical Engineering
  • Separation Science
  • Proteomics

Background:

  • Isoelectric focusing (IEF) is a powerful protein separation technique.
  • Scaling IEF from analytical to preparative levels presents challenges.
  • Understanding the underlying mechanisms is crucial for optimizing preparative IEF.

Purpose of the Study:

  • To adapt free-flow isoelectric focusing (FFIF) for preparative-scale protein separations.
  • To apply chemical engineering principles to elucidate FFIF mechanisms.
  • To validate experimental findings with a numerical model.

Main Methods:

  • Free-flow isoelectric focusing apparatus utilized for separation.
  • Mixture of human serum albumin (pI 4.6) and beta-lactoglobulin (pI 5.22) used as sample.

Related Experiment Videos

  • pH gradients generated using carrier ampholytes across various ranges (4-6.5, 3.5-5, 4-5.5, 4.5-5.0).
  • Numerical modeling employed to simulate and validate separation.

    • Main Results:

    • Successful separation of human serum albumin and beta-lactoglobulin achieved.
    • Optimal separation performance observed within the pH 4-5.5 gradient range.
    • Experimental results demonstrated strong agreement with the numerical model predictions.

    Conclusions:

    • Free-flow isoelectric focusing is a viable method for preparative protein separation.
    • Chemical engineering approaches provide valuable insights into FFIF operational mechanisms.
    • Numerical modeling serves as an effective tool for predicting and validating FFIF performance.