[Molecular-genetic analysis of the R2 plasmid (group IncP-9)]

The IncP-9 test plasmid R2 controlling resistance to streptomycin, carbenicillin, sulfanylamides and UV irradiation was studied. The plasmid (67.5 kb) was physically mapped for restriction endonucleases EcoRI, BamHI, BglII and SmaI. The aphC, sulI and bla genes were localized on the physical map of the plasmid. The resistance genes are supposed to be comprised within the ANT-R element. The regions participating in replication and mobilization of this plasmid were cloned. The cloned oriV region of the plasmid mediated synthesis of the protein with molecular weight of about 36 kD.