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Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)
Published on: May 20, 2013
High-performance liquid chromatographic method for the determination of polycyclic hydrocarbon metabolite
Sugiyanto1, G M Holder, A J Ryan
1Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, Indonesia.
Abstract:
Analytical separations and quantification of eight metabolites of benzo[a]pyrene, nine metabolites of 7-methylbenz[c]acridine and nine metabolites of dibenz[a,j]acridine have been carried out on two reversed-phase high-performance liquid chromatographic systems using 3- and 10-microns C8 packings. Quantitative results for the distributions of primary metabolites for the two systems closely agreed and showed that up to 70% savings in chromatographic time could be achieved with the 3 micron particle size columns.
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