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Isolation of Catharanthus alkaloids by solid-phase extraction and semipreparative HPLC
T Naaranlahti1, M Nordström, S P Lapinjoki
1Department of Pharmaceutical Chemistry, University of Kuopio, Finland.
Journal of Chromatographic Science
|April 1, 1990
Abstract:
A combination of moderate-pressure chromatography on C18 sorbent and preparative HPLC is developed for rapid isolation of alkaloids from Catharanthus roseus. The procedure is optimized for vindoline and catharanthine with respective yields of 3 and 2 mg per 1 g of dried leaves of the plant. The methodology is also applied for identification of the above and other alkaloids from cultured plant cells.