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A Fast and Reliable Pipeline for Bacterial Transcriptome Analysis Case study: Serine-dependent Gene Regulation in Streptococcus pneumoniae
Published on: April 25, 2015
Microarray analysis of temperature-induced transcriptome of Streptococcus suis serotype 2
Xiaotao Zeng1, Yuan Yuan, Yan Wei
1State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing, China.
Abstract:
Streptococcus suis serotype 2 (S. suis S2) is able to cause human infections ranging from superficial wounded skin infections to severe invasive infections such as meningitis and streptococcal toxic shock-like syndrome. During its infection cycle, S. suis S2 must acclimatize itself to temperature shift. Herein, a whole-genome DNA microarray was used to investigate the global transcriptional regulation of an invasive strain of S. suis S2 grown to late-exponential phase at 29°C or 40°C relative to 37°C. The differentially regulated genes that were detected included those encoding virulence factors, antigenic proteins, ATP-binding-cassette transporters, and proteins of unknown functions. Our data provided a global profile of gene transcription induced by temperature alteration and shed light on some unforeseen lines for further pathogenesis investigation.
Insights
Streptococcus suis serotype 2 (S. suis S2) alters gene expression in response to temperature changes. This study reveals how S. suis S2 adapts to host environments, impacting its virulence.
Area of Science:
- Microbiology
- Genomics
- Infectious Diseases
Background:
- Streptococcus suis serotype 2 (S. suis S2) causes significant human infections, from skin issues to severe conditions like meningitis.
- S. suis S2 must adapt to temperature shifts during infection to survive and cause disease.
Purpose of the Study:
- To investigate the global transcriptional regulation of S. suis S2 in response to temperature changes.
- To identify genes involved in S. suis S2 adaptation and pathogenesis.
Main Methods:
- Whole-genome DNA microarray analysis was employed.
- Gene expression profiles of S. suis S2 were compared at 29°C and 40°C relative to 37°C.
Main Results:
- Significant differences in gene transcription were observed at different temperatures.
- Key genes encoding virulence factors, antigenic proteins, and ATP-binding-cassette transporters were differentially regulated.
- Proteins of unknown function were also identified as being temperature-responsive.
Conclusions:
- Temperature alteration significantly impacts the global gene transcription of S. suis S2.
- The findings provide insights into S. suis S2 adaptation mechanisms and potential new avenues for investigating its pathogenesis.
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