Mono-sulfated glycosphingolipids of human amnion

Two mono-sulfated glycosphingolipids were isolated from the human amnion. By carbohydrate analysis and acid hydrolysis, the structure of these glycolipids are proposed to be galactosylsulfatide (SM4s) and lactosylsulfatide (SM3). The major fatty acids of SM4s and SM3 were C16:0. The yields of SM4s and SM3 from the amnion were about 340 and 110 pmol/g wet wt, respectively.