Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Lipids as Anchors

Lipids as Anchors

In the plasma membrane, the lipids forming the bilayer can also act as an anchor to tether proteins to the membrane. The three main types of lipid anchors found in eukaryotes are – prenyl groups, fatty acyl groups, and glycosylphosphatidylinositol or GPI groups. Prenyl and fatty acyl groups act as anchors on the cytosolic surface of the membrane, whereas GPI anchors proteins on the extracellular side.
The carboxy-terminal of most of the prenylated proteins, such as Ras proteins, contains the...

Lipid Digestion

Lipid Digestion

Lipids are large molecules that are generally not water-soluble. Since most of the digestive enzymes in the human body are water-based, there are specific steps the body must take to break down lipids and make them available for use.

Formation of Lipopolysaccharides

Formation of Lipopolysaccharides

Lipopolysaccharides (LPS) are crucial components of the outer membrane of Gram-negative bacteria, serving both structural and functional roles. It contributes to membrane stability and protects bacteria from host immune responses. LPS is composed of three major regions—lipid A, a core oligosaccharide, and an O antigen. The biosynthesis and assembly of LPS involve a highly coordinated set of enzymatic reactions and transport mechanisms. Additionally, LPS is recognized as an endotoxin, triggering...

Lipid Catabolism

Lipid Catabolism

Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...

Biosynthesis of Lipids

Biosynthesis of Lipids

Microbial membranes exhibit remarkable diversity in lipid composition, reflecting evolutionary adaptations to various environmental conditions. The three domains of life—Bacteria, Archaea, and Eukarya—synthesize membrane lipids through distinct biosynthetic pathways, leading to fundamental structural differences that impact membrane stability, function, and adaptability.Fatty Acid-Based Lipids in Bacteria and EukaryaBacteria and eukaryotes share a common fatty acid biosynthesis pathway, which...

Lipid Absorption

Lipid Absorption

Dietary triglycerides from chyme in the duodenum are mixed with bile salts produced by the liver to emulsify fats. As a result, large droplets are broken down into smaller ones, increasing the surface area for enzymatic action. Once emulsified, pancreatic lipases hydrolyze the triglycerides into free fatty acids and monoglycerides.
These breakdown products bind with bile salts and lecithin to form micelles, which quickly pass between microvilli to come in close contact with the apical...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A Novel PSEN1 K311R Mutation Discovered in Chinese Families with Late-Onset Alzheimer's Disease Affects Amyloid-β Production and Tau Phosphorylation.

Journal of Alzheimer's disease : JAD·2017

Same author

Progesterone/estradiol ratio <0.25 on the day of human chorionic gonadotropin administration is associated with adverse pregnancy outcomes in prolonged protocols for in vitro fertilization/intracytoplasmic sperm injection.

Taiwanese journal of obstetrics & gynecology·2017

Same author

Ascorbic acid metabolism during sweet cherry (Prunus avium) fruit development.

PloS one·2017

Same author

Consensus-based recommendations for the management of rapid cognitive decline due to Alzheimer's disease.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2017

Same author

Enzyme-Catalyzed Intramolecular Enantioselective Hydroalkoxylation.

Journal of the American Chemical Society·2017

Same author

Risk assessment of aggressive behavior in Chinese patients with schizophrenia by fMRI and <i>COMT</i> gene.

Neuropsychiatric disease and treatment·2017

Search research articles

Related Experiment Video

Updated: Jun 9, 2026

Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry

Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry

Published on: May 21, 2018

Adding the lipo to lipopeptides: do more with less.

Yit-Heng Chooi¹, Yi Tang

¹Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA.

Chemistry & Biology

|August 28, 2010

Summary

This summary is machine-generated.

Surfactin, a lipopeptide antibiotic, has its potency influenced by its lipid chain. Understanding lipoinitiation is key for future modifications of these important antibiotics.

More Related Videos

Mechanical Micronization of Lipoaspirates for Regenerative Therapy

Mechanical Micronization of Lipoaspirates for Regenerative Therapy

Published on: March 15, 2019

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes

Published on: March 3, 2020

Related Experiment Videos

Last Updated: Jun 9, 2026

Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry

Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry

Published on: May 21, 2018

Mechanical Micronization of Lipoaspirates for Regenerative Therapy

Mechanical Micronization of Lipoaspirates for Regenerative Therapy

Published on: March 15, 2019

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes

Published on: March 3, 2020

Area of Science:

Biochemistry
Microbiology
Medicinal Chemistry

Background:

Surfactin belongs to the lipopeptide antibiotic family, similar to the drug daptomycin.
Antibiotic potency is determined by the lipid chain attached to the peptidyl backbone.

Discussion:

Lipoinitiation is the process where the lipid chain is incorporated into the nonribosomally assembled peptidyl backbone.
This mechanism is crucial for the biological activity of lipopeptide antibiotics.

Key Insights:

The study by Kraas et al. (2010) offers significant insights into the lipoinitiation mechanism.
This understanding is vital for elucidating how lipopeptide antibiotics function.

Outlook:

Insights into lipoinitiation can guide future biosynthetic modifications of lipopeptide antibiotics.
This research may lead to the development of novel or improved antibiotic therapies.