Related Experiment Video
Updated: Jun 9, 2026

Drug-induced Sensitization of Adenylyl Cyclase: Assay Streamlining and Miniaturization for Small Molecule and siRNA Screening Applications
Published on: January 27, 2014
Synthesis of cyclic di-nucleotidic acids as potential inhibitors targeting diguanylate cyclase
Shi Min Ching1, Wan Jun Tan, Kim Lee Chua
1Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore.
Abstract:
Five analogs of cyclic di-nucleotidic acid including c-di-GMP were synthesized and evaluated for their biological activities on Slr1143, a diguanylate cyclase of Synechocystis sp. Slr1143 was overexpressed from the recombinant plasmid which contained the gene of interest and subsequently purified by affinity chromatography. A new HPLC method capable of separating the compound and product peaks with good resolution was optimized and applied to the analysis of the compounds. Results obtained show that cyclic di-inosinylic acid 1b demonstrates a stronger inhibition on Slr1143 than c-di-GMP and is a potential inhibitor for biofilm formation.
More Related Videos
Related Concept Videos
GPCRs Regulate Adenylyl Cylase Activity
Two...
GTPases and their Regulation
Large G-proteins, also known...
Inhibition of Cdk Activity
Inhibition of CDK Activity
IP3/DAG Signaling Pathway
Biosynthesis of Nucleic Acids

