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Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Chromatin Structure Regulates pre-mRNA Processing

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...

Regulation of Nuclear Protein Sorting

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...

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Related Experiment Video

Updated: Jun 9, 2026

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

The nuclear-retained noncoding RNA MALAT1 regulates alternative splicing by modulating SR splicing factor

Vidisha Tripathi¹, Jonathan D Ellis, Zhen Shen

¹Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.

|August 28, 2010

Summary

The nuclear-retained regulatory RNA MALAT1 regulates gene expression by influencing alternative splicing. MALAT1 modulates the levels of active serine/arginine splicing factors, impacting transcriptome complexity.

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Last Updated: Jun 9, 2026

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

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Published on: April 10, 2018

Using the E1A Minigene Tool to Study mRNA Splicing Changes

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Published on: April 22, 2021

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

Area of Science:

Molecular Biology
RNA Biology
Gene Regulation

Background:

Alternative splicing (AS) increases transcriptome and proteomic complexity in eukaryotes.
Serine/arginine (SR) splicing factors control cell-type-specific AS, but regulatory mechanisms are unclear.

Purpose of the Study:

To investigate the role of the long nuclear-retained regulatory RNA (nrRNA) MALAT1 in alternative splicing regulation.
To elucidate how MALAT1 influences the activity of SR splicing factors.

Main Methods:

Studied the interaction between MALAT1 and SR proteins.
Assessed the impact of MALAT1 depletion and SR protein overexpression on AS.
Analyzed the distribution of splicing factors in nuclear speckles.
Measured cellular levels of phosphorylated SR proteins.

Main Results:

MALAT1 interacts with SR proteins and affects their distribution in nuclear speckles.
Depletion of MALAT1 or alteration of SR protein levels impacts the AS of common pre-mRNAs.
MALAT1 influences the phosphorylation status of SR proteins, affecting their activity.
MALAT1 regulates cellular levels of active SR proteins.

Conclusions:

MALAT1 acts as a regulator of alternative splicing by modulating the levels of active SR proteins.
This study highlights the significant role of nrRNAs in the intricate regulation of gene expression.