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Related Experiment Video

Updated: Jun 9, 2026

High Throughput Measurement of Extracellular DNA Release and Quantitative NET Formation in Human Neutrophils In Vitro

High Throughput Measurement of Extracellular DNA Release and Quantitative NET Formation in Human Neutrophils In Vitro

Published on: June 18, 2016

Extracellular DNA traps promote thrombosis.

Tobias A Fuchs¹, Alexander Brill, Daniel Duerschmied

¹Immune Disease Institute, Boston, MA 02115, USA.

Proceedings of the National Academy of Sciences of the United States of America

|August 28, 2010

Summary

Neutrophil extracellular traps (NETs) form a scaffold that promotes blood clot (thrombus) formation. Inhibiting NETs or histones prevents this, revealing a link between inflammation and thrombosis.

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Stimulation of Vascular Endothelial Cells Using Neutrophil Extracellular Traps in the Presence of Low-Density Lipoprotein

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Published on: August 12, 2025

Area of Science:

Immunology
Vascular Biology
Hematology

Background:

Neutrophil extracellular traps (NETs) are DNA-based structures involved in innate immunity.
NETs are increasingly recognized in non-infectious diseases and within the vasculature.

Purpose of the Study:

To investigate the role of NETs in thrombus formation.
To explore the mechanisms by which NETs contribute to thrombosis.

Main Methods:

Perfusing blood over NETs.
Using DNase and heparin to dismantle NETs.
Stimulating platelets with purified histones.
Analyzing thrombus composition and markers in vivo.

Main Results:

NETs induced platelet adhesion, activation, and aggregation.
DNase and heparin inhibited NET-induced thrombus formation.
Histones alone were sufficient to aggregate platelets.
NETs promoted red blood cell recruitment and fibrin deposition, forming red thrombi.
NET markers were detected in deep vein thrombosis models.

Conclusions:

NETs serve as a scaffold and stimulus for thrombus formation.
NETs represent a novel link between inflammation and thrombosis.
This finding may explain the association between infection and thrombosis.