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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

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Updated: Jun 9, 2026

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

Nanopore DNA sequencing with MspA.

Ian M Derrington¹, Tom Z Butler, Marcus D Collins

¹Department of Physics, University of Washington, Seattle, WA 98195-1560, USA.

Proceedings of the National Academy of Sciences of the United States of America

|August 28, 2010

Summary

This summary is machine-generated.

This study shows that the Mycobacterium smegmatis porin A (MspA) nanopore can distinguish individual DNA nucleotides. This demonstrates a simple, fast, and inexpensive DNA sequencing method using nanopore technology.

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Last Updated: Jun 9, 2026

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Published on: December 14, 2017

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

Area of Science:

Biotechnology
Genomics
Molecular Biology

Background:

Nanopore sequencing offers a promising approach for rapid and cost-effective DNA analysis.
Current nanopore sequencing methods rely on detecting ionic current changes as DNA passes through a pore.

Purpose of the Study:

To investigate the potential of Mycobacterium smegmatis porin A (MspA) as a nanopore for DNA sequencing.
To demonstrate the ability of MspA to differentiate individual DNA nucleotides.

Main Methods:

Utilizing an engineered MspA nanopore to analyze single-stranded DNA.
Measuring ionic current modulation as DNA nucleotides pass through the MspA pore constriction.
Employing double-stranded DNA segments to temporarily position nucleotides within the pore.

Main Results:

The MspA nanopore successfully distinguished between all four DNA nucleotides.
Single-nucleotide resolution was achieved for single-stranded DNA.
Proof of principle for a simplified nanopore DNA sequencing method was established.

Conclusions:

MspA exhibits the capability to serve as a functional nanopore for DNA sequencing.
The MspA nanopore is a significant development for advancing nanopore sequencing technologies.
This research highlights MspA's potential impact on the future of rapid and affordable DNA sequencing.