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Related Experiment Video

Updated: Jun 9, 2026

Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources

Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources

Published on: September 3, 2009

Resolving postglacial phylogeography using high-throughput sequencing.

Kevin J Emerson¹, Clayton R Merz, Julian M Catchen

¹Center for Ecology and Evolutionary Biology, University of Oregon, Eugene, OR 97403-5289, USA.

Proceedings of the National Academy of Sciences of the United States of America

|August 28, 2010

Summary

This summary is machine-generated.

Researchers used restriction site-associated DNA tags (RAD tags) to uncover genetic structure in the pitcher plant mosquito. This method reveals evolutionary history in nonmodel organisms, aiding phylogeography and speciation studies.

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Novel Sequence Discovery by Subtractive Genomics

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

Area of Science:

Evolutionary Biology
Genomics
Ecology

Background:

The line between model and nonmodel organisms is blurring in biological research.
High-throughput sequencing is increasingly applied to diverse species based on unique traits, not just genetic data availability.

Purpose of the Study:

To demonstrate a low-cost, efficient technique for fine-scale phylogenetic analysis in recently diverged populations.
To investigate the genetic structure and evolutionary direction in the pitcher plant mosquito (Wyeomyia smithii).

Main Methods:

Application of restriction site-associated DNA tags (RAD tags) for genetic analysis.
Phylogenetic analysis of Wyeomyia smithii populations from a southern Appalachian Mountain refugium.

Main Results:

Unresolved genetic structure and evolutionary direction were revealed using RAD tags.
Detailed phylogeographic patterns were identified in Wyeomyia smithii populations.

Conclusions:

The RAD tag method is effective for studying phylogeography in any organism, irrespective of genomic data.
This technique can identify incipient speciation and genome-wide variation in natural populations.