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Donors leukapheresis on Fenwal CS-3000.
B Kotelba-Witkowska1, Z R Mrowiec, J Daszyński
1Instytut Hematologii, Warszawa, Poland.
Materia Medica Polona. Polish Journal of Medicine and Pharmacy
|January 1, 1990
Summary
Comparing two sedimentation agents for leukocyte collection revealed no significant differences in product yield or donor hematologic parameters. Both Dextran 110 and hydroxyethyl starch (HES) are effective, though leukocyte and platelet counts decrease post-procedure.
Area of Science:
- Hematology
- Transfusion Medicine
- Cell Separation Technology
Background:
- Leukocyte collection is crucial for various medical applications.
- Optimizing collection efficiency and donor safety is paramount.
- Citric acid-based sedimentation agents are commonly used.
Purpose of the Study:
- To compare the efficacy of Dextran 110 and hydroxyethyl starch (HES) as sedimentation agents for leukocyte collection.
- To evaluate the impact of these agents on product yield and donor hematologic parameters.
Main Methods:
- Leukocytes were collected from 24 donors using a Fenwal CS-3000 cell separator.
- Two sedimentation agents in citric acid, Dextran 110 and HES, were utilized.
- Donor hematologic data were evaluated post-collection.
Main Results:
- No significant differences in leukocyte product yield were observed between Dextran 110 and HES.
- Donor blood tests showed no significant variations between the two agents.
- Average donor leukocyte count decreased by 30% post-procedure.
- Mean donor platelet count decreased by an average of 23%.
Conclusions:
- Both Dextran 110 and HES are effective sedimentation agents for leukocyte collection.
- The choice between Dextran 110 and HES does not significantly impact product yield or donor hematologic safety.
- Observed decreases in leukocyte and platelet counts are consistent with hemodilution effects.