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Validated method for the quantification of atractylenolide III in different processed products of rhizoma
Qinhua Chen1, Peng Li, Fang Zheng
1Dongfeng Hospital,Yunyang Medical College, Hubei, 442008, People's Republic of China.
Introduction:
Rhizoma Atractylodes Macrocephalae (RAM) contains several sesquiterpene compounds including atractylenolide III (AO-III). This bioactive compound may be used as a chemical marker for the quality control of different processed RAM products.
Objective:
To develop and validate an RP-HPLC method for the quantitative determination of AO-III in RAM and in a variety of processed RAM products.
Methodology:
HPLC was carried out using a Kromssil C(18) RP-column eluted with methanol-water (70:30) at a flow rate of 1.0 mL/min and with UV detection at 220 nm. Full validation was performed using standard methods.
Results:
The linear range of AO-III was 5-50 µg/mL; the regression equation was y = 10210x + 11194 (r = 0.9994) and the average recovery was 101.08% (RSD = 0.98%). The detection and quantification limits for AO-III were determined to be 0.005 and 0.018 µg/mL at signal-to-noise ratios of approximately 3:1 and 10:1, respectively.
Conclusion:
The described HPLC method is appropriate for quality assurance and differentiation of AO-III in RAM and different processed products.
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