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Related Concept Videos

Computed Tomography01:10

Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...

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Related Experiment Video

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Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
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Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Published on: February 12, 2014

Lifetime-based tomographic multiplexing.

Scott B Raymond1, David A Boas, Brian J Bacskai

  • 1The Harvard-MIT Division of Health Sciences and Technology, Charlestown, Massachusetts 02129, USA.

Journal of Biomedical Optics
|August 31, 2010
PubMed
Summary
This summary is machine-generated.

This study introduces fluorescence lifetime-based separation for in vivo near-infrared (NIR) tomography, enabling imaging of multiple, spectrally overlapping fluorophores. The technique successfully distinguishes fluorophores in mice, advancing multi-target biological imaging.

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Area of Science:

  • Biomedical Optics
  • Molecular Imaging
  • Fluorescence Tomography

Background:

  • Near-infrared (NIR) fluorescence tomography is crucial for in vivo imaging but limited by spectral overlap of fluorophores.
  • Existing methods struggle to differentiate multiple NIR fluorophores due to broad emission spectra and limited spectral bandwidth.

Purpose of the Study:

  • To develop and validate a novel method for in vivo tomography of multiple spectrally overlapping NIR fluorophores.
  • To demonstrate the utility of fluorescence lifetime-based separation for multiplexed imaging in biological systems.

Main Methods:

  • Utilized time-domain fluorescence tomography with a voltage-gated intensified CCD and Ti:sapphire laser excitation.
  • Employed fluorescence lifetime component fitting from decay curves and reconstructed images using a lifetime-adjusted forward model.
  • Tested commercially available fluorophores for in vivo lifetime multiplexing suitability in solution and in nude mice.

Main Results:

  • Demonstrated quantitative separation of two spectrally overlapping fluorophores with distinct lifetimes (1.1 ns and 1.37 ns) at a 1:5 concentration ratio.
  • Confirmed narrow in vivo fluorescence lifetime distributions for tested fluorophores, suitable for lifetime multiplexing.
  • Successfully performed tomographic imaging of two and three NIR fluorophores in nude mice, localizing them to different organ systems.

Conclusions:

  • Fluorescence lifetime-based separation is a viable technique for overcoming spectral limitations in multi-fluorophore NIR tomography.
  • This method enables accurate in vivo imaging and differentiation of multiple NIR fluorophores, with broad applicability in biomedical research.
  • The developed system shows promise for advanced 3-D molecular imaging and diagnostics in preclinical models.