Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 9, 2026

Mesoscopic Optical Imaging of Whole Mouse Heart

Mesoscopic Optical Imaging of Whole Mouse Heart

Published on: October 14, 2021

Three-dimensional optical method for integrated visualization of mouse islet microstructure and vascular network with

Ya-Yuan Fu¹, Chih-Hsuan Lu, Chi-Wen Lin

¹National Tsing Hua University, Department of Chemical Engineering, Hsinchu, Taiwan.

Journal of Biomedical Optics

|August 31, 2010

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Vertically stacked immobilized photocatalyst devices towards land-efficient solar hydrogen production.

Nature communications·2026

Same author

Individualized decision-making driven by long-term memory retrieval in Drosophila.

Cell reports·2026

Same author

Self-Supervised Isotropic Resolution Enhancement of Expansion Microscopy via Quantized Compression.

Journal of imaging informatics in medicine·2026

Same author

Emerging applications of insect (protein, lipid) and insect waste (chitosan, frass, exuviae) -derived materials in sustainable technologies: A review.

International journal of biological macromolecules·2025

Same author

Delayed, transient, lateralized deficits in pattern separation and pattern completion after focal hippocampal irradiation in humans.

Scientific reports·2025

Same author

Redox regulation of memory formation by Rrp1 in <i>Drosophila</i>.

Proceedings of the National Academy of Sciences of the United States of America·2025

Optical clearing enables 3D imaging of mouse pancreatic islets, revealing microstructures and vasculature. This advanced microscopy technique overcomes tissue opacity for better understanding of islet physiology in normal and diabetic conditions.

Area of Science:

Endocrinology
Microscopy
Biomedical Imaging

Background:

Understanding islet of Langerhans pathophysiology requires microscopic visualization.
Pancreatic tissue opacity limits in situ high-resolution light microscopy.
2D tissue analysis restricts islet architecture visualization.

Purpose of the Study:

To develop an optical clearing method for enhanced 3D imaging of mouse pancreatic islets.
To visualize islet microstructure and vasculature in situ with subcellular resolution.
To overcome limitations of traditional 2D tissue analysis for islet assessment.

Main Methods:

Applied optical clearing to mouse pancreatic tissue to reduce light scattering.
Utilized vessel painting with lipophilic dye for islet vasculature labeling.

More Related Videos

Stabilized Longitudinal In Vivo Cellular-Level Visualization of the Pancreas in a Murine Model with a Pancreatic Intravital Imaging Window

Stabilized Longitudinal In Vivo Cellular-Level Visualization of the Pancreas in a Murine Model with a Pancreatic Intravital Imaging Window

Published on: May 6, 2021

Computer-assisted Large-scale Visualization and Quantification of Pancreatic Islet Mass, Size Distribution and Architecture

Computer-assisted Large-scale Visualization and Quantification of Pancreatic Islet Mass, Size Distribution and Architecture

Published on: March 4, 2011

Related Experiment Videos

Last Updated: Jun 9, 2026

Mesoscopic Optical Imaging of Whole Mouse Heart

Mesoscopic Optical Imaging of Whole Mouse Heart

Published on: October 14, 2021

Stabilized Longitudinal In Vivo Cellular-Level Visualization of the Pancreas in a Murine Model with a Pancreatic Intravital Imaging Window

Stabilized Longitudinal In Vivo Cellular-Level Visualization of the Pancreas in a Murine Model with a Pancreatic Intravital Imaging Window

Published on: May 6, 2021

Computer-assisted Large-scale Visualization and Quantification of Pancreatic Islet Mass, Size Distribution and Architecture

Computer-assisted Large-scale Visualization and Quantification of Pancreatic Islet Mass, Size Distribution and Architecture

Published on: March 4, 2011

Employed confocal microscopy and voxel-based processing for 3D visualization.

Main Results:

Optical clearing enabled penetrative, 3D microscopic imaging of islets.
Revealed clear, continuous optical sections of islet microstructure and vasculature.
Achieved subcellular-level resolution in 3D islet imaging.

Conclusions:

Optical clearing provides a novel imaging approach for 3D visualization of pancreatic islets.
This method enhances understanding of islet physiology beyond conventional planar views.
Offers a valuable tool for studying islet structure and vascularization in various physiological states.