Related Experiment Video
Updated: Jun 9, 2026

Direct Detection of Isolevuglandins in Tissues Using a D11 scFv-Alkaline Phosphatase Fusion Protein and Immunofluorescence
Published on: July 5, 2021
Appraisal of translocation pathways for displaying ankyrin repeat protein on phage particles
Sawitree Nangola1, Philippe Minard, Chatchai Tayapiwatana
1Division of Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand. sawitree2727@hotmail.com
Abstract:
Depending on the molecular properties of the proteins of interest (POI), the rate of success in displaying proteins on phage particles is unpredictable. Formation of polypeptide tertiary structure in the cytoplasm occasionally results in low level display on viral particles. Here we assessed the influence of different leader peptides on the display of a premature cytoplasmic folding protein, ankyrin repeat protein (ARP), via the minor coat protein pIII. These peptides include the Sec, SRP and Tat pathways. The results demonstrated that the Sec and SRP pathways were capable of displaying the protein on the viral particle, whereas the Tat pathway failed to do so. Interestingly, the Tat pathway efficiently directed ARP through its translocon without fusing with pIII. Furthermore, the soluble form of ARP was detected in Escherichia coli periplasm.
Related Concept Videos
Directing Proteins to the Rough Endoplasmic Reticulum
Conservative Site-specific Recombination and Phase Variation
The recognition sites for Cre recombinase called LoxP...
Bacterial Translocation and Protein Secretion
Tail-anchoring of Proteins in the ER Membrane

