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Effects of anticoagulants on human plasma soluble corin levels measured by ELISA

Ningzheng Dong1, Jing Dong, Peng Liu

  • 1Cyrus Tang Hematology Center, Jiangsu Institute of Hematology, First Affiliated Hospital, Soochow University, Suzhou, China. ningzheng_dong@yahoo.com.cn

Clinica Chimica Acta; International Journal of Clinical Chemistry
|August 31, 2010
PubMed

Insights

Soluble corin levels in plasma are stable but vary with anticoagulant. Heparin yielded higher corin measurements than EDTA or sodium citrate, impacting heart failure biomarker research.

Area of Science:

  • Biochemistry
  • Cardiovascular Research
  • Clinical Diagnostics

Background:

  • Soluble corin has been identified in human plasma.
  • Lower plasma corin levels are observed in heart failure patients compared to healthy individuals.
  • Standardized measurement of plasma or serum corin is crucial for accurate clinical assessment.

Purpose of the Study:

  • To optimize and standardize the immunoassay for measuring soluble corin in human plasma and serum.
  • To investigate the impact of different anticoagulants on plasma corin quantification.
  • To establish reference ranges for plasma corin levels in a healthy population.

Main Methods:

  • Enzyme-linked immunosorbent assay (ELISA) was employed to quantify corin levels.
  • The influence of various anticoagulants (EDTA, heparin, sodium citrate) on plasma corin concentrations was evaluated.
  • Corin stability was assessed under different storage conditions (4°C, freeze-thaw) in plasma with EDTA.

Main Results:

  • Serum corin levels were comparable to those in heparinized plasma but significantly higher than in plasma treated with EDTA or sodium citrate.
  • Human corin protein demonstrated stability in EDTA-treated plasma at 4°C and after freeze-thaw cycles.
  • In 348 healthy Chinese individuals, plasma corin levels ranged from 216 to 1663 pg/ml, with significantly higher levels observed in males (842±283 pg/ml) than females (569±192 pg/ml).

Conclusions:

  • Soluble corin is stable in plasma samples, making it suitable for diagnostic assays.
  • Anticoagulant choice significantly affects plasma corin measurements, with heparin preserving higher levels.
  • These findings are critical for accurate interpretation of corin levels in cardiovascular studies and diagnostics.
Abstract

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