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Updated: Jun 9, 2026
06:52
Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages
Published on: May 21, 2018
[Inflammasome and cardiovascular diseases].
1Laboratoire de physiopathologie et pharmacologie cardiovasculaires expérimentales, IFR Santé-STIC, faculté de médecine, université de Bourgogne, Dijon, France. connatjl@u-bourgogne.fr
Annales De Cardiologie Et D'Angeiologie
|August 31, 2010
Summary
NOD-like receptors (NLRs) form inflammasomes that activate inflammation. This study shows NLRP3 inflammasomes play a key role in atherosclerosis development and progression.
Area of Science:
- Immunology
- Cardiovascular Biology
- Molecular Medicine
Background:
- NOD-like receptors (NLRs) are intracellular pattern recognition receptors.
- NALP subfamily NLRs form inflammasomes, activating inflammation via caspase-1 and cytokines IL-1β and IL-18.
Purpose of the Study:
- To review the roles of NLRs in pathologies.
- To investigate the role of inflammasomes in cardiovascular diseases, specifically atherosclerosis.
Main Methods:
- Literature review on NLRs and pathologies.
- Immunocytochemistry to detect Nlrp3 inflammasome expression and activation in atherosclerotic plaques and specific monocyte subsets.
- Analysis of oxLDL modulation in murine macrophages.
Main Results:
- Nlrp3 inflammasome is expressed in atherosclerotic plaques.
- Nlrp3 inflammasome is upregulated and activated in a specific atherosclerosis-prone monocyte subset.
- Oxidized low-density lipoprotein (oxLDL) modulates Nlrp3 inflammasome in macrophages.
Conclusions:
- NLRP3 inflammasomes mature atherogenic cytokines IL-1β and IL-18.
- NLRP3 inflammasome has an unexpected role in the development of atherosclerosis.
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