Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are slanted or...
¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)01:15

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prognostic Value of Inflammatory and Metabolic Biomarkers in ICU-Admitted Trauma Patients: A Retrospective Cohort Study.

Medicina (Kaunas, Lithuania)·2025
Same author

Can serum orexin levels be used as a marker in childhood epilepsy?

Heliyon·2025
Same author

Carbon capture in polymer-based electrolytes.

Science advances·2024
Same author

Stabilization of T<sub>2</sub> relaxation and magnetization transfer in cartilage explants by immersion in perfluorocarbon liquid.

Magnetic resonance in medicine·2019
Same author

Facial Herpes Zoster Following Rhinoplasty: A Rare Complication.

Aesthetic surgery journal·2018
Same author

Corrigendum to "Combination therapy with lenalidomide and nanoceria ameliorates CNS autoimmunity", [Exp. Neurol. 273 (2015), 151-160].

Experimental neurology·2016
Same journal

X-band EPR with bilateral twins of composite magnets.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

Localization-driven exchange contrast in diffusion exchange spectroscopy.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

4.5 Tesla superconducting miniature magnet in liquid nitrogen.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

Folding and unfolding dynamics of a DNA aptamer studied by heteronuclear <sup>1</sup>H-<sup>13</sup>C correlation zz-exchange spectroscopy.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

Multi-spin control from one-spin pulses.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

Altering MRI rotating frame relaxations by changing the truncation level of Hyperbolic Secant pulse.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
See all related articles

Related Experiment Video

Updated: Jun 9, 2026

NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins
09:25

NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins

Published on: November 1, 2024

Filter diagonalization using a "sensitivity-enhanced basis": improved performance for noisy NMR spectra.

Hasan Celik1, A J Shaka

  • 1Department of Chemistry, University of California, Irvine, CA 92697-2025, USA. hcelik@uci.edu

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|August 31, 2010
PubMed
Summary
This summary is machine-generated.

This study enhances the Filter Diagonalization Method (FDM) for processing NMR data. Modified FDM significantly improves noise performance, offering better spectral estimates from noisy or incomplete datasets.

Related Experiment Videos

Last Updated: Jun 9, 2026

NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins
09:25

NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins

Published on: November 1, 2024

Area of Science:

  • Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Computational Chemistry
  • Data Processing

Background:

  • The Filter Diagonalization Method (FDM) is useful for processing NMR data, especially with sparse spectra and sharp lines.
  • Noise can degrade spectral resolution and frequency accuracy in FDM estimates.
  • Paradoxically, more complete data can worsen FDM spectra in the presence of noise.

Purpose of the Study:

  • To improve the noise performance of the Filter Diagonalization Method (FDM) for NMR data processing.
  • To maintain the existing advantages of FDM while enhancing its robustness against noise.
  • To provide a modified FDM suitable for general-purpose use with noisy and incomplete NMR data.

Main Methods:

  • Adjusting FDM basis functions to minimize noise contribution in matrix elements.
  • Independently regularizing each dimension of multidimensional NMR spectra.
  • Implementing numerical modifications to the FDM algorithm.

Main Results:

  • Significant improvement in FDM noise performance was achieved.
  • The modifications did not compromise the existing advantages of the FDM.
  • Enhanced FDM provides better spectral estimates from noisy and incomplete NMR data.

Conclusions:

  • The modified FDM offers superior noise handling capabilities for NMR spectral analysis.
  • These modifications make FDM more robust and suitable for a wider range of experimental conditions.
  • The enhanced FDM is recommended for processing somewhat noisy, incomplete NMR data.