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Related Experiment Video

Updated: Jun 9, 2026

Cell Fractionation of U937 Cells by Isopycnic Density Gradient Purification
10:19

Cell Fractionation of U937 Cells by Isopycnic Density Gradient Purification

Published on: August 12, 2021

Proteomic differentiation pattern in the U937 cell line.

Luigi Minafra1, Gianluca Di Cara, Nadia Ninfa Albanese

  • 1Dipartimento di Oncologia Sperimentale ed Applicazioni Cliniche, Università di Palermo, Palermo, Italy.

Leukemia Research
|August 31, 2010
PubMed
Summary
This summary is machine-generated.

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This study reveals proteomic changes during U937 cell differentiation into macrophages, identifying new markers for hematological diseases. These findings advance our understanding of monocyte/macrophage differentiation.

Area of Science:

  • Hematology
  • Proteomics
  • Cell Biology

Background:

  • The U937 cell line is a key in vitro model for hematological studies.
  • U937 cells can differentiate into macrophage-like cells upon stimulation with factors like 12-O-tetradecanoyl-13-phorbol acetate (TPA).
  • The molecular mechanisms underlying monocyte/macrophage differentiation in U937 cells are not fully understood.

Purpose of the Study:

  • To identify a proteomic-based differentiation pattern in TPA-induced U937 cells.
  • To elucidate the molecular basis of U937 cell differentiation into macrophage-like cells.
  • To discover potential protein markers for myelo-monocytic differentiation.

Main Methods:

  • Utilized proteomic analysis to compare protein expression profiles of U937 cells before and after TPA treatment.

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Last Updated: Jun 9, 2026

Cell Fractionation of U937 Cells by Isopycnic Density Gradient Purification
10:19

Cell Fractionation of U937 Cells by Isopycnic Density Gradient Purification

Published on: August 12, 2021

Cell Fractionation of U937 Cells in the Absence of High-speed Centrifugation
06:53

Cell Fractionation of U937 Cells in the Absence of High-speed Centrifugation

Published on: January 18, 2019

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  • Quantified and identified differentially expressed proteins.
  • Correlated proteomic changes with the acquisition of macrophage-like phenotype.

    • Main Results:

    • U937 cell differentiation induced by TPA resulted in significant proteomic modulation affecting approximately 30% of identified proteins.
    • Proteins involved in cell proliferation regulation and metabolic processes were downregulated.
    • Proteins associated with phagocytic activity and calcium/phospholipid binding were upregulated, indicating a shift towards a macrophagic phenotype.

    Conclusions:

    • TPA-induced differentiation of U937 cells leads to substantial proteomic alterations.
    • The identified differentially expressed proteins may serve as valuable markers for myelo-monocytic differentiation.
    • These markers could be applied to the study of various hematological diseases.