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Affinity sensor using 3-aminophenylboronic acid for bacteria detection.

Rodtichoti Wannapob1, Proespichaya Kanatharana, Warakorn Limbut

  • 1Trace Analysis and Biosensor Research Center, Center for Innovation in Chemistry, Department of Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.

Biosensors & Bioelectronics
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PubMed
Summary

This study presents a novel biosensor for detecting bacteria using boronic acid that binds to bacterial cell walls. This cost-effective method offers rapid and reliable bacterial detection in various water samples.

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Area of Science:

  • Biosensor development
  • Analytical chemistry
  • Microbiology

Background:

  • Bacteria possess diol-groups on their cell walls, which can be targeted for detection.
  • Boronic acids exhibit reversible binding affinity towards diols, enabling specific molecular recognition.

Purpose of the Study:

  • To develop a sensitive and reusable biosensor for bacterial detection.
  • To utilize the affinity of boronic acid for diols on bacterial surfaces for quantitative analysis.
  • To assess the applicability of the developed sensor for real-world water sample analysis.

Main Methods:

  • Immobilization of 3-aminophenylboronic acid (3-APBA) on a gold electrode surface.
  • Detection of bacterial binding via changes in surface capacitance using a potentiostatic step method.
  • Regeneration and reuse of the modified electrode for multiple detection cycles.

Main Results:

  • Achieved a linear detection range of 1.5x10^2 to 1.5x10^6 CFU/ml with a detection limit of 1.0x10^2 CFU/ml.
  • Demonstrated sensor reusability for up to 58 cycles.
  • Validated the method's accuracy against the standard plate count method across diverse water types (bottled, well, tap, reservoir, wastewater).

Conclusions:

  • The 3-APBA modified electrode provides a rapid, cost-effective, and reusable platform for bacterial detection.
  • The sensor technology shows promise for analyzing bacteria in various water matrices.
  • The underlying principle can be extended to develop affinity sensors for other cis-diol-containing compounds.