Related Experiment Video
Updated: Jun 9, 2026
08:51
Magnetic Resonance Imaging of Multiple Sclerosis at 7.0 Tesla
Published on: February 19, 2021
Responsive Mn(II) complexes for potential applications in diagnostic Magnetic Resonance Imaging
Gabriele A Rolla1, Lorenzo Tei, Marianna Fekete
1Dipartimento di Scienze dell'Ambiente e della Vita, Università degli Studi del Piemonte Orientale Amedeo Avogadro, Viale T. Michel 11, 15121 Alessandria, Italy.
Bioorganic & Medicinal Chemistry
|August 31, 2010
Summary
New manganese (Mn(II))-based imaging probes detect tyrosinase, an enzyme overexpressed in melanoma. These probes show significant relaxation enhancement, paving the way for novel melanoma diagnostic tools.
Area of Science:
- Biomedical Imaging
- Chemical Biology
- Medical Diagnostics
Background:
- Tyrosinase enzyme expression is elevated in melanoma cells.
- Developing targeted molecular imaging probes is crucial for cancer diagnostics.
- Manganese (II)-based complexes offer potential as contrast agents for Magnetic Resonance Imaging (MRI).
Purpose of the Study:
- To design and characterize novel Mn(II)-based probes responsive to tyrosinase.
- To evaluate the diagnostic potential of these probes for melanoma detection.
- To elucidate the mechanism of action for tyrosinase-responsive imaging agents.
Main Methods:
- Synthesis and characterization of three novel Mn(II) complexes with modified polyaminocarboxylate ligands incorporating l-tyrosine.
- (1)H NMR relaxometry to assess probe characteristics in aqueous media.
- Evaluation of tyrosinase responsiveness by monitoring relaxivity changes in buffered solutions and melanoma cell homogenates.
Main Results:
- The developed Mn(II) complexes exhibited significant responsive activity to tyrosinase.
- Relaxation enhancement ranged from 50% for MnL(1) to 350% for MnL(3).
- Magnetic field-dependent relaxivity and Electron Spin Resonance (ESR) spectra provided insights into the mechanism of action.
Conclusions:
- Novel Mn(II)-based probes demonstrate tyrosinase-responsive imaging capabilities.
- These probes show promise for the development of advanced diagnostic tools for melanoma.
- The study offers new understanding into the mechanism of tyrosinase-responsive MRI contrast agents.
Related Concept Videos
01:24Magnetic Resonance Imaging
Magnetic resonance imaging (MRI) is a noninvasive medical imaging technique based on a phenomenon of nuclear physics discovered in the 1930s, in which matter exposed to magnetic fields and radio waves was found to emit radio signals. In 1970, a physician and researcher named Raymond Damadian noticed that malignant (cancerous) tissue gave off different signals than normal body tissue. He applied for a patent for the first MRI scanning device in clinical use by the early 1980s. The early MRI...
01:21Imaging Studies for Cardiovascular System IV: CMRI
Cardiovascular magnetic resonance imaging, or CMRI, is a non-invasive diagnostic test that employs a magnetic field and radiofrequency waves to create precise images of the heart and arteries. It provides comprehensive information about cardiac anatomy, function, perfusion, and tissue characterization without ionizing radiation.IndicationsCMRI diagnoses various heart conditions, including tissue damage from heart attacks, ischemic heart disease, myocarditis, aortic issues (tears, aneurysms,...
01:25Applications Of NMR In Biology
Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
The...
The...
01:14Imaging Studies I: CT and MRI
Introduction: MRI and CT scans are crucial advancements in medical imaging techniques, playing a vital role in diagnosing conditions related to the gastrointestinal (GI) system. Each scan serves distinct purposes, targets specific areas, and requires unique nursing duties.
Description of the Procedures
Computed Tomography (CT) scan:
Computed Tomography (CT) scans use X-ray technology to generate detailed images of bones, organs, and tissues. During the scan, the patient lies on a moving table...
Description of the Procedures
Computed Tomography (CT) scan:
Computed Tomography (CT) scans use X-ray technology to generate detailed images of bones, organs, and tissues. During the scan, the patient lies on a moving table...
01:27Imaging Studies IV: Magnetic Resonance Imaging
Introduction:Magnetic Resonance Imaging, or MRI, can include a specialized imaging technique of the urinary system known as Magnetic Resonance Urography (MRU). This radiation-free technique uses strong magnetic fields and radio waves to produce detailed images with the help of a computer. MRU is particularly effective for visualizing fluid-filled structures like the kidneys, ureters, and bladder.Applications of MRI in the Genitourinary SystemKidneys and Ureters: MRI detects tumors, cysts,...