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Updated: Jun 9, 2026

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
Published on: February 1, 2018
Engineered lactic acid bacterium Lactococcus lactis capable of binding antibodies and tumor necrosis factor alpha
Matjaz Ravnikar1, Borut Strukelj, Natasa Obermajer
1Department of Biotechnology, JoŽef Stefan Institute, Jamova 39, SI-1000 Ljubljana, Slovenia. ales.berlec@ijs.si.
Abstract:
We have optimized the display of the B domain of staphylococcal protein A on the surface of Lactococcus lactis. The maximum binding capacity was estimated at 0.146 μg of antibody per 10⁸ cells and was sustained at 86% after treatment with simulated gastric juice. A tumor necrosis factor alpha (TNF-α)-binding affibody was also displayed and bound TNF-α, which could be useful in the treatment of inflammatory bowel disease.
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