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Changes of gene expression during long term adaptation of human EUE cells to a hypertonic medium: electrophoretic
M G Manfredi Romanini1, A Fraschini, A M Fuhrman Conti
1Dipartimento di Biologia Animale and Centro di Studio per l'Istochimica del C.N.R., Pavia, Italy.
Acta Histochemica. Supplementband
|January 1, 1990
Abstract:
The effects of long term (1 to 10 days) growth in a hypertonic medium have been studied in human EUE cells. Following polyacrylamide gel electrophoresis, a change in the protein pattern has been found, with the progressive enhancement, during adaptation, of a 33 kDa band. Experiments of DNA digestion in situ by DNase I showed that chromatin DNA of cells grown in a hypertonic medium is more available to the enzyme cleavage. These findings show that long term hypertonic stress is able to induce a change in gene expression in EUE cells.