Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Bidirectional fluorescent labelling techniques for the developing and regenerating visual system.

S Thanos1

  • 1Max-Planck-Institut für Entwicklungsbiologie und Augenklinik, Medizinischen Fakultät, Universität Tübingen, FRG.

Acta Histochemica. Supplementband
|January 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Topographic protein profiling of the age-related proteome in the retinal pigment epithelium of Callithrix jacchus with respect to macular degeneration.

Journal of proteomics·2018
Same author

Charge transfer excitations from excited state Hartree-Fock subsequent minimization scheme.

The Journal of chemical physics·2014
Same author

[Impact of age on success rate of lacrimal duct irrigation with silicone tube intubation in connatal lacrimal duct stenosis].

Klinische Monatsblatter fur Augenheilkunde·2013
Same author

Dysfunctional uveoscleral pathway in a rat model of congenital glaucoma.

Journal of physiology and pharmacology : an official journal of the Polish Physiological Society·2013
Same author

Double excitations from modified Hartree Fock subsequent minimization scheme.

The Journal of chemical physics·2013
Same author

Everolimus improves experimental autoimmune uveoretinitis.

Experimental eye research·2012
Same journal

[The distribution of intermediate filaments in intracranial vessels in humans].

Acta histochemica. Supplementband·1992
Same journal

[Malignant angioendotheliomatosis as a rare cause of cerebrovascular insufficiency].

Acta histochemica. Supplementband·1992
Same journal

[The effect of arteriosclerosis on the wall elasticity of the human common carotid artery].

Acta histochemica. Supplementband·1992
Same journal

[Vascular etiopathogenesis and degenerative dementias--current state of knowledge].

Acta histochemica. Supplementband·1992
Same journal

Histochemical and morphometric investigation of the pathogenesis of acute brain infarction in primates.

Acta histochemica. Supplementband·1992
Same journal

[Current aspects in diagnosis of Parkinson disease].

Acta histochemica. Supplementband·1992
See all related articles

Fluorescent dyes like RITC and Dil are effective for labeling developing and regenerating neurons, allowing visualization of axons, growth cones, cell bodies, and dendrites. These dyes are non-toxic, persistent, and useful for studying neural development and regeneration.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Biochemistry

Background:

  • Investigating neuronal development and regeneration requires effective labeling techniques.
  • Fluorescent dyes offer a method for visualizing neuronal structures and pathways.

Purpose of the Study:

  • To evaluate the utility of RITC and Dil fluorescent dyes for labeling neurons.
  • To explore the anterograde and retrograde transport capabilities of these dyes in neurons.

Main Methods:

  • Utilized RITC and Dil fluorescent dyes for neuronal labeling.
  • Observed dye uptake by cell bodies and transport along axons and dendrites.
  • Examined dye persistence and neurotoxicity in labeled neurons.

Main Results:

Related Experiment Videos

  • RITC and Dil efficiently labeled neuronal cell bodies, axons, growth cones, and dendrites via anterograde and retrograde transport.
  • Dyes demonstrated poor solubility in aqueous buffers but were non-toxic and persisted long-term in neurons.
  • Dil proved effective for post-mortem neuronal staining.

Conclusions:

  • RITC and Dil are valuable tools for studying neuronal development, regeneration, and pathfinding.
  • These dyes facilitate detailed investigation of axonal growth and guidance during embryogenesis and adult regeneration.