Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Central Dogma01:25

The Central Dogma

Overview
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
From DNA to Protein03:06

From DNA to Protein

The flow of genetic information in cells from DNA to mRNA to protein is described by the central dogma, which states that genes specify the sequence of mRNAs, which in turn specify the sequence of amino acids making up all proteins. The decoding of one molecule to another is performed by specific proteins and RNAs. Because the information stored in DNA is so central to cellular function, it makes intuitive sense that the cell would make mRNA copies of this information for protein synthesis...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MINTsC learns multi-way chromatin interactions from single cell high throughput chromatin conformation data.

Nature communications·2026
Same author

BRD4 recruitment desilences transcription without erasure or depletion of repressive chromatin.

bioRxiv : the preprint server for biology·2026
Same author

Label-Free High-Throughput Screening of CYP3A4 Inhibitors Using Acoustic Ejection Mass Spectrometry.

Analytical chemistry·2026
Same author

MINTsC learns multi-way chromatin interactions from single cell high throughput chromatin conformation data.

bioRxiv : the preprint server for biology·2026
Same author

Investigating the role of FKBP4 in testis germ cell tumor via integrated in silico approach.

In silico pharmacology·2026
Same author

Systematic background selection with BasCoD enhances contrastive dimension reduction in single cell genomics.

Nature communications·2026

Related Experiment Video

Updated: Jun 9, 2026

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping
07:00

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

Published on: May 25, 2015

Chemical-genomic dissection of the CTD code.

Joshua R Tietjen1, David W Zhang, Juan B Rodríguez-Molina

  • 1Department of Biochemistry and The Genome Center, University of Wisconsin, Madison, Wisconsin, USA.

Nature Structural & Molecular Biology
|August 31, 2010
PubMed
Summary

RNA polymerase II C-terminal domain (CTD) modifications coordinate transcription. The study reveals Ser7-P marks are placed early by Bur1 kinase, aiding transcription elongation and suppressing cryptic transcription.

More Related Videos

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
14:06

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Published on: November 12, 2012

Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions
11:42

Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions

Published on: September 30, 2016

Related Experiment Videos

Last Updated: Jun 9, 2026

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping
07:00

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

Published on: May 25, 2015

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
14:06

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Published on: November 12, 2012

Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions
11:42

Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions

Published on: September 30, 2016

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Biochemistry

Background:

  • RNA polymerase II C-terminal domain (CTD) undergoes sequential phosphorylation.
  • CTD modifications dictate the recruitment and release of transcription machinery.
  • Understanding CTD mark dynamics is crucial for deciphering transcription regulation.

Purpose of the Study:

  • To map the genome-wide distribution of early (Ser5-P), mid (Ser7-P), and late (Ser2-P) CTD marks.
  • To investigate the temporal placement and function of Ser7-P marks during transcription.
  • To explore the role of the CTD kinase Bur1 in Ser7-P deposition.

Main Methods:

  • Genome-wide mapping of CTD phosphorylation marks (Ser5-P, Ser7-P, Ser2-P).
  • Chemical-genomic analysis to probe kinase activity and mark placement.
  • Analysis of CTD mark patterns across different gene classes.

Main Results:

  • Widespread co-occurrence of Ser5-P, Ser7-P, and Ser2-P marks was observed.
  • Contrary to previous understanding, Ser7-P marks are deposited early and persist until transcription termination.
  • Promoter-distal Ser7-P marks are actively placed by Bur1 kinase, not remnants.
  • High Ser7-P levels correlate with highly transcribed genes, suggesting a role in elongation.

Conclusions:

  • Ser7-P modification is an early event in transcription, placed by Bur1 kinase.
  • Ser7-P marks play a role beyond noncoding RNA processing, potentially facilitating transcription elongation.
  • The findings suggest Ser7-P may help suppress cryptic transcription initiation.