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Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Related Experiment Video

Updated: Jun 9, 2026

Measuring Intracellular Ca2+ Changes in Human Sperm using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging
19:26

Measuring Intracellular Ca2+ Changes in Human Sperm using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging

Published on: May 24, 2013

Laser fluorescence spectroscopy from human spermatozoa.

G C Tang, N Oka, G R Nagamatsu

    Applied Optics
    |August 31, 2010
    PubMed
    Summary

    This study investigated the native fluorescence of spermatozoa, including sperm heads and tails. Findings reveal how fluorescence properties depend on sperm density and laser excitation.

    Area of Science:

    • Biophysics
    • Spectroscopy
    • Cell Biology

    Background:

    • Spermatozoa possess intrinsic fluorescence properties.
    • Understanding these properties is crucial for sperm analysis and diagnostics.
    • Laser excitation offers a non-invasive method for probing cellular components.

    Purpose of the Study:

    • To characterize the native fluorescence spectra of spermatozoa.
    • To investigate the influence of sperm density on fluorescence intensity.
    • To determine relaxation decay times and polarization of sperm components.

    Main Methods:

    • Laser excitation was used to analyze spermatozoa.
    • Native fluorescence spectra were measured.
    • Intensity dependence on sperm density was evaluated.

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    Fluorescence in situ hybridization (FISH) Protocol in Human Sperm

    Published on: September 1, 2009

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    Last Updated: Jun 9, 2026

    Measuring Intracellular Ca2+ Changes in Human Sperm using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging
    19:26

    Measuring Intracellular Ca2+ Changes in Human Sperm using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging

    Published on: May 24, 2013

    Fluorimetric Techniques for the Assessment of Sperm Membranes
    08:58

    Fluorimetric Techniques for the Assessment of Sperm Membranes

    Published on: November 28, 2018

    Fluorescence in situ hybridization (FISH) Protocol in Human Sperm
    16:19

    Fluorescence in situ hybridization (FISH) Protocol in Human Sperm

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  • Relaxation decay time and polarization were determined.

    • Main Results:

    • Native fluorescence spectra of spermatozoa were successfully measured.
    • Fluorescence intensity showed a dependence on sperm density.
    • Relaxation decay times and polarization characteristics were elucidated for sperm heads and tails.

    Conclusions:

    • Native fluorescence provides valuable biophysical information about spermatozoa.
    • Sperm density significantly impacts observable fluorescence.
    • Detailed characterization of sperm components' fluorescence is achievable using laser excitation.