Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Time-Resolved Fluorescence Intensity and Anisotropy Decays of 2,5-Diphenyloxazole by Two-Photon Excitation and Frequency-Domain Fluorometry.

The Journal of physical chemistry·2019
Same author

Surface-confined living radical polymerization for coatings in capillary electrophoresis.

Analytical chemistry·2011
Same author

Electrostatic Interactions between Ru(bpy)(3)(2+) and Chromatographic Surfaces.

Analytical chemistry·2011
Same author

Spectroscopic Probing of Mixed-Mode Adsorption of Ru(bpy)(3)(2+) to Silica.

Analytical chemistry·2011
Same author

Chemical analysis in small domains: introduction.

Chemical reviews·2001
Same author

Specification of neuropeptide Y phenotype in visual cortical neurons by leukemia inhibitory factor.

Development (Cambridge, England)·2000
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: Jun 9, 2026

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
09:19

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Published on: August 29, 2025

Detector nonlinearity in frequency-domain fluorometry.

M J Wirth, J D Burbage, S L Zulli

    Applied Optics
    |August 31, 2010
    PubMed
    Summary
    This summary is machine-generated.

    Conventional photomultipliers can introduce intensity-dependent phase shifts in frequency-domain fluorometry. This finding is critical for accurate fluorescence anisotropy measurements, as it affects signal interpretation.

    More Related Videos

    Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle
    15:06

    Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

    Published on: January 3, 2016

    A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
    08:43

    A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

    Published on: December 1, 2018

    Related Experiment Videos

    Last Updated: Jun 9, 2026

    Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
    09:19

    Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

    Published on: August 29, 2025

    Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle
    15:06

    Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

    Published on: January 3, 2016

    A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
    08:43

    A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

    Published on: December 1, 2018

    Area of Science:

    • Photophysics and Spectroscopy
    • Fluorescence Measurement Techniques

    Background:

    • Frequency-domain fluorometry measures fluorescence signals using phase and amplitude analysis.
    • Accurate measurements are crucial for techniques like fluorescence anisotropy.

    Purpose of the Study:

    • To investigate potential artifacts in frequency-domain fluorometry.
    • To evaluate the linearity and phase response of conventional photomultipliers.

    Main Methods:

    • Experimental measurements of photomultiplier response under varying light intensities.
    • Analysis of phase shifts in the fluorescence signal.

    Main Results:

    • Conventional photomultipliers exhibit intensity-dependent phase shifts.
    • These phase shifts occur at light intensities well below the photomultiplier's maximum linear current.
    • The parallel and perpendicular emission components in anisotropy experiments can be differentially affected.

    Conclusions:

    • Photomultiplier-induced phase shifts pose a significant challenge for accurate frequency-domain fluorometry.
    • This artifact can lead to errors in fluorescence anisotropy measurements.
    • Careful consideration of photomultiplier behavior is necessary for reliable experimental data.