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[Collagen as a carrier for heart cell cultures]
W Schulze1, G Wallukat, A Altankow
1Zentralinstitut für Herz-Kreislauf-Forschung, Akademie der Wissenschaften, DDR, Berlin-Buch.
Abstract:
Extracellular collagen from rat tail (types 1 and 3) is a suitable matrix for isolated neonatal heart cell cultivation. The fine structure and the physiological response to isoproterenol for instance is similar for neonatal cardiomyocytes cultured in the medium Sm 20-1 with or without collagen matrix. For electron microscopical routine preparation it is easier to use matrix-grown cardiomyocytes because additional preparation steps (centrifugation) are unnecessary.