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The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective

The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective

Published on: June 16, 2022

Microfiltration conditions modify Lactobacillus bulgaricus cryotolerance in response to physiological changes.

Fernanda Streit¹, Violaine Athès, Amine Bchir

¹UMR 782 Génie et Microbiologie des Procédés Alimentaires, AgroParisTech, INRA CBAI, Thiverval-Grignon, France.

Bioprocess and Biosystems Engineering

|August 31, 2010

Summary

Microfiltration enhances Lactobacillus bulgaricus CFL1 cryotolerance by optimizing membrane fluidity. Specific conditions improve bacterial resistance during freezing and frozen storage, crucial for starter culture quality.

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Area of Science:

Food Microbiology
Bioprocess Engineering
Dairy Science

Background:

Lactobacillus bulgaricus CFL1 is a key starter culture in dairy fermentation.
Maintaining bacterial viability during freezing and frozen storage is critical for starter culture efficacy.
Traditional concentration methods like centrifugation may impact bacterial cell integrity.

Purpose of the Study:

To investigate the impact of microfiltration conditions on the quality of frozen Lactobacillus bulgaricus CFL1 starters.
To determine the optimal microfiltration parameters for enhanced bacterial cryotolerance.
To elucidate the relationship between microfiltration, membrane composition, and cell survival.

Main Methods:

Pilot-scale microfiltration of Lactobacillus bulgaricus CFL1.
Varying cross-flow velocity and transmembrane pressure during microfiltration.
Assessing bacterial resistance during concentration, freezing, and frozen storage.
Analyzing bacterial membrane fatty acid composition.

Main Results:

Microfiltered cells showed reduced resistance during concentration compared to centrifuged cells.
Bacterial cryotolerance improved by 28-88% post-microfiltration, dependent on conditions.
Optimal cryotolerance achieved at 2 m/s cross-flow velocity and 0.15 MPa transmembrane pressure.
Improved cryotolerance correlated with increased unsaturated and cyclic fatty acid ratios in cell membranes.

Conclusions:

Microfiltration is a viable method to enhance Lactobacillus bulgaricus CFL1 cryotolerance.
Specific microfiltration conditions (2 m/s, 0.15 MPa) significantly improve bacterial survival during freezing and storage.
Enhanced membrane fluidity, due to altered fatty acid composition, is key to improved cryotolerance.