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Updated: Jun 9, 2026

Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure
Published on: May 27, 2022
Rapid identification of yeasts from positive blood culture bottles by pyrosequencing
I Quiles-Melero1, J García-Rodriguez, M P Romero-Gómez
1Servicio de Microbiología, Hospital Universitario La Paz-IdiPAZ, Paseo de La Castellana, Madrid, Spain.
Abstract:
We have developed a rapid protocol for the identification of Candida species from positive blood cultures by combining a simple method for nucleic acid extraction and preparation using microbial storage cardboards with polymerase chain reaction (PCR) and pyrosequencing of a small region of the 18 S rRNA gene. The protocol is robust and easy to implement and can be performed in 4 h. The method was tested against a collection of clinical blood cultures. Agreement of sequence identifications with standard microbiological methods was 100%.
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