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A doubly signal-amplified DNA detection method based on pre-complexed [Ru(bpy)3]2+-doped silica nanoparticles

Se Won Bae1, Min Sun Cho, Seong-Suk Hur

  • 1Department of Chemistry, Seoul National University, Seoul 151-747, Korea.

Chemistry (Weinheim an Der Bergstrasse, Germany)
|August 31, 2010
PubMed

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...