Study on secondary metabolite content of Helleborus niger L. leaves

Phytochemical investigation of Helleborus niger L. (Ranunculaceae) leaf methanol extract allowed to isolate a phenolic glucoside derivative and two flavonoid glycosides characterized as phenyllactic acid 2-O-β-D-glucopyranoside (1), quercetin 3-O-2-(E-caffeoyl)-α-L-arabinopyranosyl-(1 → 2)-β-D-glucopyranoside-7-O-β-D-glucopyranoside (2), and kaempferol 3-O-α-L-arabinopyranosyl-(1 → 2)-β-D-galactopyranoside-7-O-β-D-glucopyranoside (3), respectively. Compounds 1 and 2 were isolated for the first time and their structural characterization was obtained on the basis of extensive NMR spectral studies.