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Updated: Jun 9, 2026

Improved Genome Editing via Oviductal Nucleic Acids Delivery-based In Vivo Electroporation Technique for Knockout Mice Generation
Published on: August 26, 2025
Analysis of microRNA knockouts in mice
Chong Y Park1, Yun S Choi, Michael T McManus
1UCSF Diabetes Center, University of California-San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143-0534, USA.
Abstract:
MicroRNAs (miRNAs) are small noncoding RNAs that act as potent regulators of gene expression. The discovery of miRNAs with specific temporal and spatial expression patterns revealed a hidden layer of post-transcriptional gene regulation. Furthermore, differential expression of miRNAs during disease progression identified miRNAs as relevant candidate genes in human pathologies. Currently the exact roles of miRNAs in human development and disease progression remain largely unknown. There have been recent efforts to study the loss of these genes in vivo and this review will discuss published miRNA knockout mouse models, highlighting their potential mechanisms of action in vivo.
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