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Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
Eukaryotic Transcription Inhibitors01:52

Eukaryotic Transcription Inhibitors

Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a DNA...
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...

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Related Experiment Video

Updated: Jun 9, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

MEF/ELF4 transactivation by E2F1 is inhibited by p53.

Manabu Taura1, Mary Ann Suico, Ryosuke Fukuda

  • 1Department of Molecular Medicine, Graduate School of Pharmaceutical Sciences, Global COE 'Cell Fate Regulation Research and Education Unit', Kumamoto University, Kumamoto 862-0973, Japan.

Nucleic Acids Research
|September 1, 2010
PubMed
Summary

The tumor suppressor p53 inhibits myeloid elf-1-like factor (MEF) by blocking E2F1 binding to the MEF promoter. This reveals a novel negative regulatory mechanism between p53 and MEF in cellular senescence.

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Purification of Ubiquitinated p53 Proteins from Mammalian Cells
10:55

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Cancer Research

Background:

  • Myeloid elf-1-like factor (MEF), an ETS-related transcription factor, downregulates p53 and inhibits p53-mediated cellular senescence.
  • The reciprocal regulation of MEF by p53 has not been previously investigated.

Purpose of the Study:

  • To investigate the reciprocal regulatory relationship between p53 and MEF.
  • To elucidate the mechanism by which p53 modulates MEF expression.

Main Methods:

  • Analysis of MEF expression and promoter activity in human cells and mouse tissues.
  • Identification of transcription factor binding sites on the MEF promoter.
  • Investigation of p53 and E2F1 interaction and their effects on MEF promoter activity.
  • Assessment of MEF expression in doxorubicin-induced senescent cells.

Main Results:

  • p53 suppresses MEF expression and promoter activity.
  • E2F1 binds to the MEF promoter and transactivates MEF.
  • p53 inhibits E2F1 binding to the MEF promoter by forming a complex with E2F1.
  • Activation of p53 reduces E2F1 recruitment to the MEF promoter and decreases MEF expression.

Conclusions:

  • p53 negatively regulates MEF by inhibiting the transcriptional activator E2F1.
  • A novel negative feedback loop between p53 and MEF is identified, contributing to the regulation of cellular senescence.