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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability

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Comprehensive Analysis of Transcription Dynamics from Brain Samples Following Behavioral Experience
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Effect of long-term storage in TRIzol on microarray-based gene expression profiling.

Wencai Ma1, Michael Wang, Zhi-Qiang Wang

  • 1The Myeloma Tissue and Leukemia Satellite Sample Banks, Department of Lymphoma and Myeloma, University of Texas M D Anderson Cancer Center, Houston, Texas 77054, USA.

Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology
|September 1, 2010
PubMed
Summary
This summary is machine-generated.

Prolonged storage of samples in TRIzol does not negatively impact gene expression profiling (GEP). This method effectively preserves RNA quality, ensuring reliable results for array-based analyses like GEP.

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Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • TRIzol reagent is a common method for RNA isolation and preservation.
  • Concerns exist that prolonged TRIzol storage may interfere with gene expression profiling (GEP) due to reverse transcription issues.

Purpose of the Study:

  • To investigate the impact of prolonged TRIzol storage on array-based GEP.
  • To determine if TRIzol storage affects RNA integrity and subsequent gene expression analysis.

Main Methods:

  • Gene expression profiling (GEP) was performed on Illumina arrays using paired sample aliquots.
  • Samples were either cryopreserved or stored in TRIzol, originating from multiple myeloma (MM) and acute myeloid leukemia (AML) patients.
  • Data analysis was conducted at both probe level and bead level.

Main Results:

  • TRIzol storage did not introduce significant differentially expressed probes (DEPs) within sample groups (MM or AML).
  • While bead-level analysis revealed some DEPs, most were minor (<2-fold change), and no consistent evidence of premature reverse transcription termination was found.
  • Increased signals in TRIzol-preserved samples were observed for probes near the 5' end of transcripts, suggesting enhanced mRNA preservation.

Conclusions:

  • TRIzol is a suitable reagent for RNA preservation for GEP, maintaining RNA quality without detrimental effects.
  • Array-based GEP can be reliably performed on samples stored with or without TRIzol, with minimal concern for systematic artifacts.