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Updated: Jun 9, 2026
11:28
Imaging CD4 T Cell Interstitial Migration in the Inflamed Dermis
Published on: March 25, 2016
Cdc42 interacting protein 4 (CIP4) is essential for integrin-dependent T-cell trafficking
Suresh Koduru1, Lalit Kumar, Michel J Massaad
1Division of Immunology, Children's Hospital, Boston, MA 02115, USA.
Summary
The F-BAR domain protein CIP4 is crucial for T cell responses. Its absence impairs antibody production, germinal center formation, and effector T cell migration to infection sites.
Area of Science:
- Immunology
- Cell Biology
- Molecular Biology
Background:
- The F-BAR domain protein CIP4 (Cdc42 interacting protein 4) interacts with Cdc42 and WASP/N-WASP.
- CIP4 is implicated in filamentous actin assembly.
- Integrins play a key role in T cell migration and immune responses.
Purpose of the Study:
- To investigate the role of CIP4 in T cell-dependent immune responses.
- To elucidate the function of CIP4 in germinal center formation and effector T cell recruitment.
Main Methods:
- Analysis of CIP4 knockout (CIP4(-/-)) mice.
- Assessment of T and B lymphocyte development and antibody production.
- Evaluation of T cell migration, adhesion, and transmigration assays.
- Contact hypersensitivity (CHS) assays.
Main Results:
- CIP4(-/-) mice exhibited normal T and B lymphocyte development but impaired T cell-dependent antibody production, IgG affinity maturation, and germinal center formation.
- CIP4(-/-) mice showed defective contact hypersensitivity (CHS).
- Activated CIP4(-/-) T cells displayed impaired adhesion and polarization on VCAM-1 and ICAM-1, and defective transmigration across endothelial cells.
Conclusions:
- CIP4 plays a critical role in integrin-dependent T cell-dependent antibody responses and germinal center formation.
- CIP4 is essential for the integrin-mediated recruitment of effector T cells to cutaneous sites during immune reactions.
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