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Updated: Jun 9, 2026

In Vitro Polymerization of F-actin on Early Endosomes
Published on: August 28, 2017
Endosomal-sorting complexes required for transport (ESCRT) pathway-dependent endosomal traffic regulates the
Chun Tu1, Cesar F Ortega-Cava, Paul Winograd
1Eppley Institute for Research in Cancer and Allied Diseases and University of Nebraska Medical Center-Eppley Cancer Center, University of Nebraska Medical Center, Omaha, NE 68198, USA.
Abstract:
Active Src localization at focal adhesions (FAs) is essential for cell migration. How this pool is linked mechanistically to the large pool of Src at late endosomes (LEs)/lysosomes (LY) is not well understood. Here, we used inducible Tsg101 gene deletion, TSG101 knockdown, and dominant-negative VPS4 expression to demonstrate that the localization of activated cellular Src and viral Src at FAs requires the endosomal-sorting complexes required for transport (ESCRT) pathway. Tsg101 deletion also led to impaired Src-dependent activation of STAT3 and focal adhesion kinase and reduced cell migration. Impairment of the ESCRT pathway or Rab7 function led to the accumulation of active Src at aberrant LE/LY compartments followed by its loss. Analyses using fluorescence recovery after photo-bleaching show that dynamic mobility of Src in endosomes is ESCRT pathway-dependent. These results reveal a critical role for an ESCRT pathway-dependent LE/LY trafficking step in Src function by promoting localization of active Src to FAs.
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