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Updated: Jun 9, 2026
Murine Colitis Modeling using Dextran Sulfate Sodium (DSS)
Published on: January 19, 2010
Effect of Arctium lappa L. in the dextran sulfate sodium colitis mouse model
Tzou-Chi Huang1, Shinn-Shyong Tsai, Li-Fang Liu
1Graduate Institute of Animal Vaccine Technology, National Pingtung University of Science and Technology, 912 Pingtung, Taiwan, China.
Aim:
To analyze the possible protective role of Arctium lappa L. (AL) in a murine model of ulcerative colitis (UC).
Methods:
BALB/c mice were administered 100 mg/kg AL powder orally each day. After 7 d, colitis was induced by administration of dextran sulfate sodium (DSS) (5% W/V) in drinking water for a further 8 consecutive days. Diarrhea and bloody stools as well as colonic histology were observed. The level of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) in colonic sections were detected by immunohistochemistry.
Results:
There were significant differences in mean body weight values and disease activity indices between controls and AL-treated animals. Moreover, the histological findings showed that AL treatment can prevent mucosal edema, submucosal erosions, ulceration, inflammatory cell infiltration and colon damage. In addition, immunohistochemistry analysis showed that the levels of the inflammatory cytokines, IL-6 and TNF-alpha were also decreased in AL-treated groups.
Conclusion:
We suggest that AL can prevent intestinal damage and decrease inflammatory cytokines in mice with DSS-induced colitis. Thus, AL could prove to be a useful food for UC.
