[Protein analysis of Trichomonas vaginalis treated by Pulsatilla chinensis in vitro]

Water extract of Pulsatilla chinensis (PWE) (0.625 mg/ml) was added to the medium for the culture of Trichomonas vaginalis. After 2 h and 4 h treatment, the content and component of soluble proteins in the parasites were analyzed by SDS-PAGE. A total of 28 protein bands was demonstrated. After treated with PWE for 2 h, the content of 5 protein bands increased and that of 4 bands decreased significantly. Four hours later, a new protein band appeared in the electrophoretogram, and the content of 5 bands increased and that of other 5 bands decreased.