Structural analysis and molecular modeling of the RvH2-e functional unit of Rapana venosa hemocyanin
Ludmila Velkova1, Pavlina Dolashka, Aleksander Dolashki
1Institute of Organic Chemistry, Bulgarian Academy of Sciences, G. Bonchev 9, Sofia 1113, Bulgaria.
Abstract:
Rapana venosa hemocyanin (RvH), a circulating glycoprotein of the marine snail, has a complex structure. To provide details on the stability of the protein, one functional unit, RvH2-e, was compared with the native molecule and the structural subunits, RvH1 and RvH2, via pH-T diagrams, typical phase portraits for stability and denaturation reversibility. By analyzing the T transition curves of RvH2-e at different pH values, several parameters of the thermodynamic functions were obtained. Increasing the temperature from 25°C to 55°C, the reversibility of the molecule of protein also increases, opening a reversibility window within the range of pH 4.0-8.0. On analyzing the pH transition curves, the start of the acid denaturation (below pH 6) and alkaline denaturation (above pH 9) was determined to be between 20°C and 35°C. For this range, the thermodynamic functions ΔH° and ΔG° for a standard temperature of 25°C were calculated.
More Related Videos
08:18Methodology for Studying Interactions of Vitamin A Membrane Receptors and Opsin Protein with their Ligands in Generating the Retinylidene Protein
Published on: October 4, 2024
07:38Comprehensive Echocardiographic Assessment of Right Ventricle Function in a Rat Model of Pulmonary Arterial Hypertension
Published on: January 20, 2023
Related Concept Videos
Globular and Fibrous Proteins
Globular proteins are also known as spheroproteins and typically are approximately round in shape. They contain a mix of amino acid types and contain differing sequences in their primary structures. Globular proteins have many different functions, such as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be...
Gene Families
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
Protein and Protein Structure
A protein's shape is critical to its function. For example, an enzyme can...
Electron Transport Chain: Complex III and IV
Structure and Function of Erythrocytes
The erythrocyte plasma membrane is associated with proteins such as spectrin, which forms a flexible cytoplasmic meshwork. This meshwork allows erythrocytes to twist, turn, become cup-shaped, and regain their biconcave shape as they pass through narrow capillaries. Additionally, erythrocytes can form...
