Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 9, 2026

Complementation of Splicing Activity by a Galectin-3 - U1 snRNP Complex on Beads
08:48

Complementation of Splicing Activity by a Galectin-3 - U1 snRNP Complex on Beads

Published on: December 9, 2020

Mutational tuning of galectin-3 specificity and biological function.

Emma Salomonsson1, Michael C Carlsson, Veronica Osla

  • 1Section MIG, Department of Laboratory Medicine, Lund University, 223 62 Lund, Sweden.

The Journal of Biological Chemistry
|September 3, 2010
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Somatostatin receptor PET response assessment framework for patients with neuroendocrine tumours (V1.0): a modified Delphi consensus from the European Neuroendocrine Tumor Society (endorsed by EANM and NANETS).

The Lancet. Oncology·2026
Same author

An Orally Available Halothiazole Glycomimetic as a Cancer-Targeting Dual Galectin-1 and Galectin-3 Inhibitor.

Journal of medicinal chemistry·2026
Same author

Tumor microenvironment gene engineering with LOAd703 in patients with solid malignancies: LOKON002 phase I/IIb clinical trial.

Clinical cancer research : an official journal of the American Association for Cancer Research·2026
Same author

DNA methylation and expression of MAPRE3 affect overall survival of early-stage non-small cell lung cancer patients.

Molecular oncology·2026
Same author

Galectin-8 binds HIV envelope glycoproteins with high affinity and promotes viral infectivity.

Frontiers in cellular and infection microbiology·2026
Same author

Novel GH115 xylan α-1,2-glucuronidases with distinct preferences for xylan-derived oligomers and polymers.

Protein science : a publication of the Protein Society·2026

Mutating human galectin-3 altered its carbohydrate-binding fine specificity, impacting neutrophil activation and intracellular targeting. These changes reveal insights into galectin-3 function and evolution.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Immunology

Background:

  • Galectins, characterized by a conserved β-galactoside binding site, are involved in cell adhesion, signaling, and intracellular trafficking.
  • Adjacent sites modulate galectin affinity for glycans, but the biological role of this fine specificity is poorly understood.

Purpose of the Study:

  • To investigate the functional consequences of altered carbohydrate-binding fine specificity in human galectin-3.
  • To correlate specific changes in galectin-3 fine specificity with its biological functions.

Main Methods:

  • Production of 10 human galectin-3 mutants with altered adjacent binding sites.
  • Assessment of carbohydrate-binding fine specificity using glycan-array and fluorescence anisotropy assays.
  • Evaluation of mutant function in neutrophil activation and intracellular vesicle targeting assays.

More Related Videos

Modification and Functionalization of the Guanidine Group by Tailor-made Precursors
09:45

Modification and Functionalization of the Guanidine Group by Tailor-made Precursors

Published on: April 27, 2017

Related Experiment Videos

Last Updated: Jun 9, 2026

Complementation of Splicing Activity by a Galectin-3 - U1 snRNP Complex on Beads
08:48

Complementation of Splicing Activity by a Galectin-3 - U1 snRNP Complex on Beads

Published on: December 9, 2020

Modification and Functionalization of the Guanidine Group by Tailor-made Precursors
09:45

Modification and Functionalization of the Guanidine Group by Tailor-made Precursors

Published on: April 27, 2017

Main Results:

  • Mutant R186S lost affinity for LacNAc, impairing neutrophil activation and vesicle targeting.
  • Mutant K176L showed increased affinity for specific poly-N-acetyllactosaminoglycans and enhanced neutrophil activation.
  • Mutant G182A exhibited altered fine specificity and intracellular targeting, potentially linking to its known loss of anti-apoptotic effects.

Conclusions:

  • Carbohydrate-binding fine specificity is crucial for galectin-3's roles in neutrophil activation and intracellular trafficking.
  • Specific affinity changes correlate with distinct functional outcomes, highlighting the importance of glycan interactions.
  • Analysis of mutants provides insights into evolutionary differences in galectin-3 fine specificity across species.